Membrane topology of yeast alkaline ceramidase YPC1
نویسندگان
چکیده
منابع مشابه
Membrane topology of yeast alkaline ceramidase YPC1.
Ypc1p (yeast phyto-ceramidase 1) and Ydc1p (yeast dihydroceramidase 1) are alkaline ceramide hydrolases that reside in the ER (endoplasmic reticulum). Ypc1p can catalyse the reverse reaction, i.e. the condensation of non-esterified fatty acids with phytosphingosine or dihydrosphingosine and overexpression of YPC1 or YDC1 can provide enough ceramide synthesis to rescue the viability of cells lac...
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Humans and yeast possess alkaline ceramidases located in the early secretory pathway. Single deletions of the highly homologous yeast alkaline ceramidases YPC1 and YDC1 have very little genetic interactions or phenotypes. Here, we performed chemical-genetic screens to find deletions/conditions that would alter the growth of ypc1∆ydc1∆ double mutants. These screens were essentially negative, dem...
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Human alkaline ceramidase 2 (ACER2) plays an important role in cellular responses by regulating the hydrolysis of ceramides in cells. Here we report its biochemical characterization, membrane topology, and activity regulation. Recombinant ACER2 was expressed in yeast mutant cells (Deltaypc1Deltaydc1) that lack endogenous ceramidase activity, and microsomes from ACER2-expressiong yeast cells wer...
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Four proteases have been used to assess the topology of the H+-ATPase from Saccharomyces cerevisiae reconstituted into phosphatidylserine vesicles. Limited proteolysis by trypsin and alpha-chymotrypsin inactivates the enzyme and produces stable, membrane-bound fragments. Sequence analyses of these peptides have located the peptide bonds hydrolyzed. The labile bonds are on opposite sides of a ce...
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A membrane bioreactor (MBR) system based on a dead-end immersed hollow fiber membrane and filamentous fungus Aspergillus oryzae were used for treatment of baker’s yeast wastewater. The fungus was adapted to the wastewater in the bioreactor for two weeks before starting the continuous process. Average organic loading rate of 4.2 kg COD/m3.d was entered the bioreactor. MBR system was able to redu...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 2013
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj20130085